As disease and health analyses progress, the desire to monitor and analyse simpler chemical and biochemical structures has also increased. Studies have moved from the human genome to the transcriptome to the proteome until more recently the focus has been squarely on the metabolome. The human metabolome is studied with an eye to studying […]
Recently IsoSciences has focused on the manufacture and development of complete syntheses of numerous marine toxins including gonyautoxin (CAS 60508-89-6 ; 60537-65-7, tetrodotoxin (CAS 4368-28-9) and saxitoxin (CAS 35523-89-8), along with related derivatives. Availability of stable isotope labeled versions of these toxins is critical for the development of analytical and diagnostic assays by LC/MS.
Stable isotope labeling consists of using non-radioactive isotopes as tracers in biochemical systems. The most obvious benefit is the non-hazardous nature of the label and the minimized need for protective measures. There is a cost benefit associated with running a non-radioactive lab in addition to removing the fatigue and stress to personnel that occurs […]
Aldosterone (CAS 52-39-1) is a hydroxycorticosteroid hormone produced by the adrenal cortex. Due to its chemical structure, aldosterone belongs to the polycyclics and pregnenediones molecule classes. The natural synthesis of Aldosterone takes place exclusively in the outer region of the adrenal cortex, called “zona glomerulosa”, and begins with a reaction that converts cholesterol to […]
25-hydroxyvitamin-d3 (CAS 19356-17-3) is a prehormone created by the liver by the cholecalciferol 25-hydroxylase enzyme during hydroxylation of vitamin D3. It is converted in kidneys into the secosteroidhormone calcitriol, the active form of vitamin D, by the 25(OH)D-1a-hydroxylase enzyme. Similarly, it can be converted in kidneys into 24-hydroxycalcidiol through 24-hydroxylation. The name 25-hydroxyvitamin-d3 may […]
Isotope labeling is a vital tool for clarifying the flow of a particular element through a cell’s metabolic pathway. To create a stable product, a given isotopic label is fed into a cell, allowing it to grow until it reaches a steady state. Once the output pattern of the isotope labeled metabolite is determined, […]
Saxitoxin (C10H17N7O4) is among the best known neurotoxins and the most potent known sodium channel blocking agent. Saxitoxin has been called the most deadly non-protein toxin, and cannot be removed from food through standard means. Commonly produced by marine dinoflagellates or freshwater cyanobacteria and bearing paralytic properties, saxitoxin is also sometimes referred to as […]
This is a compound that is primarily used for pharmacological use. 18-Hydroxycorticosterone (CAS 561-65-9) has chemical makeup of C21H30O5, it is also known as 20-dione, 11beta, 21-trihydroxy-4-pregnene-3 among other synonyms. IIt is derived from corticosterone. It is primarily used in the synthesis of aldosterone with aldosterone synthase.
In the human body, hydroxycorticosterone is used […]
Gonyautoxin is a phytotoxin with paralyzing effects produced by several flagellate protists, called dinoflagellates. Included in the group of saxitoxin-related chemical compounds,Gonyautoxin is specifically produced by Gonyaulax catenella, Gonyaulax tamarensis, and some other Protogonyaulax species. Usually, this compound is isolated from shellfish and, along with Saxitoxin, is the neurotoxin responsible for shellfish poisoning.
This potentially […]
The use of tritium (3H) as a radio-label for tracking metabolites is a powerful tool in analytic chemistry. In fact, it is the isotope of choice for thousands of different reactions. Depending on the precursor, tritiation can be accomplished in several ways. Tritium is incorporated as radiolabel into organic molecules through a number of […]