Isotope labeling is a vital tool for clarifying the flow of a particular element through a cell’s metabolic pathway. To create a stable product, a given isotopic label is fed into a cell, allowing it to grow until it reaches a steady state. Once the output pattern of the isotope labeled metabolite is determined, it can provide important data—helping provide insight into flux reactions within a cell and rates of conversions from reactants to products.
The use of Isotope labeled metabolites has been critical to research developments in a variety of fields, including environmental analysis, bio-med, biochemistry and physics. Through the use of tracer methodology these compounds help researchers study the degradation of cells in both healthy and diseased environments. Isotope labeled metabolites are perfect for studying metabolic flux because they mimic the natural, endogenous metabolite.
Isotope labeled metabolites for pesticide research
Pesticides can produce carcinogenic pollutants into the environment like Dioxins and Furans. To study and fight the effects of Dioxin and Furan, labeled standards for each have been developed, including: Carbon 13 Chlorodioxin Standards like Dibenzo-p-dioxin (50 ± 5 µg/mL in Nonane) and Octachlorodibenzofuran (50 ± 2.5 µg/mL in Nonane). The preparation of these solutions starts with the complete synthesis of each isomer from known intermediates.
Labeled BDE Metabolites
Used in flame retardants, Brominated Diphenyl Ethers (BDE) has become a chemical of high environmental concern and study. Synthesized Carbon 13 BDEs have been used to analyze and set standards for BDE safety.
Labeled metabolites for cancer studies
As the need to investigate cancer progression at the cellular level increases, tracing and measuring labeled metabolites such as amino acids, lipids, glucose and lactate have become critical practices. The use of Carbon 12 and Carbon 13 labeled amino acids in cell cultures has furthered prostate cancer research in recent years.
It is important that any Isotope Labeled Metabolite is developed with extensive testing to ensure the utmost in isomeric purity, isotope enrichment and absolute concentration. Industry standards have been set and should be met at all times.
All metabolomic chemistries currently synthesized by IsoSciences catalog can be found here.If you are unable to find the desired metabolome in our catalog, please contact us by email or phone (610-337-3762) we will be more than happy to provide a quote on custom synthetic work.